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Caspase-3(P10) Antibody View larger

Caspase-3(P10) Antibody

ASA-B0293

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
caspase 3, apoptosis-related cysteine peptidase Polyclonal IgG Rabbit Mouse, Rat IHC-P, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse Caspase-3(P10)(220-236aa CSMLKLYAHKLEFMHIL), different from the related rat sequence by one amino acid.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 CASP3

Protein

 Caspase-3

Uniprot ID

 P42574

Function

 Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-

Tissue Specificity

Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

Sub-cellular localization

 Cytoplasm.

Sequence Similarities

 Belongs to the peptidase C14A family.

Aliases

 A830040C14Rik antibody|Apopain antibody|Apopain precursor antibody|CASP 3 antibody|CASP-3 antibody|CASP3 antibody|Casp3a antibody|Caspase 3 antibody|Caspase 3 apoptosis related cysteine protease antibody|Caspase 3 p12 subunit antibody|Caspase 3, apoptosis-related cysteine peptidase antibody|Caspase 3, apoptosis-related cysteine protease antibody|Caspase 3, apoptosis-related cysteine protease a antibody|Caspase-3 subunit p12 antibody|Caspase3 antibody|CC3 antibody|CPP 32 antibody|CPP-32 antibody|CPP32 antibody|CPP32B antibody|Cysteine protease CPP32 antibody|EC 3.4.22.56 antibody|Human cysteine protease CPP32 isoform alpha mRNA complete cds antibody|ICE3 antibody|LICE antibody|mldy antibody|OTTHUMP00000165052 antibody|OTTHUMP00000165053 antibody|OTTHUMP00000165054 antibody|PARP cleavage protease antibody|Procaspase3 antibody|Protein Yama antibody|SCA 1 antibody|SCA-1 antibody|SCA1 antibody|SREBP cleavage activity 1 antibody|Yama antibody|Yama protein antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Mouse, RatAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Rat MouseAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Caspase-3(P10) antibody, ASA-B0293, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Thymus Tissue Lysate
Lane 3: Rat Spleen Tissue Lysate
Lane 4: HEPA Cell Lysate
Lane 5: NEURO Cell Lysate
Anti-Caspase-3(P10) antibody, ASA-B0293, IHC(P)
IHC(P): Rat Intestine Tissue

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