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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| caspase 14, apoptosis-related cysteine peptidase | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Caspase-14(223-242aa KARKTNPEIQSTLRKRLYLQ), different from the related rat and mouse sequences by four amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CASP14 |
Protein | Caspase-14 |
Uniprot ID | P31944 |
Function | Non-apoptotic caspase involved in epidermal differentiation. Is the predominant caspase in epidermal stratum corneum (PubMed:15556625). Seems to play a role in keratinocyte differentiation and is required for cornification. Regulates maturation of the epidermis by proteolytically processing filaggrin (By similarity). In vitro has a preference for the substate [WY]-X-X-D motif and is active on the synthetic caspase substrate WEHD-ACF (PubMed:16854378, PubMed:19960512). Involved in processing of prosaposin in the epidermis (By similarity). May be involved in retinal pigment epithelium cell barrier function (PubMed:25121097). Involved in DNA degradation in differentiated keratinocytes probably by cleaving DFFA/ICAD leading to liberation of DFFB/CAD (PubMed:24743736). |
Tissue Specificity | Expressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum) (at protein level). Expressed in keratinocytes of hair shaft and sebaceous glands (at protein level). In psoriatic skin only expressed at very low levels (PubMed:11175259). The p17/10 mature form is expressed in epidermis stratum corneum, the p20/p8 intermediate form in epidermis upper granular cells of the stratum granulosum (PubMed:22825846). |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | |
Aliases | Apoptosis related cysteine protease antibody|CASP 14 antibody|CASP-14 antibody|CASP14 antibody|Caspase 14 apoptosis related cysteine protease antibody|Caspase 14 precursor antibody|Caspase-14 subunit p10 antibody|Caspase14 antibody|CASPE_HUMAN antibody|MGC119078 antibody|MGC119079 antibody|MICE antibody|Mini ICE antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Caspase-14 antibody, ASA-B0288, Western blotting
All lanes: Anti Caspase-14 (ASA-B0288) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: Rat Spleen Tissue Lysate at 50ug
Lane 4: A431 Whole Cell Lysate at 40ug
Lane 5: NIH3T3 Whole Cell Lysate at 40ug
Predicted bind size: 28KD
Observed bind size: 28KD
All lanes: Anti Caspase-14 (ASA-B0288) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: Rat Spleen Tissue Lysate at 50ug
Lane 4: A431 Whole Cell Lysate at 40ug
Lane 5: NIH3T3 Whole Cell Lysate at 40ug
Predicted bind size: 28KD
Observed bind size: 28KD
Anti-Caspase-14 antibody, ASA-B0288, IHC(P)
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue
Anti-Caspase-14 antibody, ASA-B0288, IHC(P)
IHC(P): Human Oesophagus Squama Cancer Tissue
IHC(P): Human Oesophagus Squama Cancer Tissue