More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| caspase 12(gene/pseudogene) | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Caspase-12(71-84aa KIFREHLWNSKKQL). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CASP12 |
Protein | Inactive caspase-12 |
Uniprot ID | Q6UXS9 |
Function | Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF. |
Tissue Specificity | Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis. |
Sub-cellular localization | |
Sequence Similarities | Belongs to the peptidase C14A family. |
Aliases | Apoptosis related cysteine protease antibody|CASP 12 antibody|CASP-12 antibody|casp12 antibody|CASP12P1 antibody|caspase 12(gene/pseudogene) antibody|caspase 12 pseudogene 1 antibody|Caspase 12 psuedogene 1 antibody|Caspase12 antibody|CASPC_HUMAN antibody|Inactive caspase-12 antibody|OTTHUMP00000207032 antibody|UNQ9415 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Caspase-12 antibody, ASA-B0287, Western blotting
All lanes: Anti Caspase-12 (ASA-B0287) at 0.5ug/ml
Lane 1: PANC Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti Caspase-12 (ASA-B0287) at 0.5ug/ml
Lane 1: PANC Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted bind size: 39KD
Observed bind size: 39KD
Anti-Caspase-12 antibody, ASA-B0287, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
Anti-Caspase-12 antibody, ASA-B0287, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue