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Caspase-1(P20) Antibody View larger

Caspase-1(P20) Antibody

ASA-B0285

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
caspase 1, apoptosis-related cysteine peptidase Polyclonal IgG Rabbit Human IHC-P, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human CASP1(135-152aa CSLEEAQRIWKQKSAEIY).

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 CASP1

Protein

 Caspase-1(CASP-1)

Uniprot ID

 P29466

Function

 Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.

Tissue Specificity

Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.

Sub-cellular localization

 Cytoplasm.

Sequence Similarities

 Belongs to the peptidase C14A family.

Aliases

 CASP 1 antibody|CASP-1 antibody|CASP1 antibody|Caspase 1 apoptosis related cysteine peptidase antibody|Caspase 1 apoptosis related cysteine protease antibody|Caspase-1 subunit p20 antibody|Caspase1 antibody|ICE antibody|IL 1 beta converting enzyme antibody|IL 1BC antibody|IL-1 beta-converting enzyme antibody|IL-1BC antibody|IL1 beta converting enzyme antibody|IL1B convertase antibody|IL1BC antibody|IL1BCE antibody|Interleukin 1 beta convertase antibody|Interleukin 1 beta convertase precursor antibody|Interleukin 1 beta converting enzyme antibody|Interleukin 1B converting enzyme antibody|Interleukin-1 beta convertase antibody|Interleukin-1 beta-converting enzyme antibody|P45 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml HumanAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml HumanAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Caspase-1(P20) antibody, ASA-B0285, Western blotting
Lane 1: JURKAT Cell Lysate
Lane 2: RAJI Cell Lysate
Lane 3: CEM Cell Lysate
Anti-Caspase-1(P20) antibody, ASA-B0285, IHC(P)
IHC(P): Human Mammary Cancer Tissue

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