More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| caspase 1, apoptosis-related cysteine peptidase | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Caspase-1(P10)(249-262aa VFIGRLIEHMQEYA). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CASP1 |
Protein | Caspase-1(CASP-1) |
Uniprot ID | P29466 |
Function | Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. |
Tissue Specificity | Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain. |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | Belongs to the peptidase C14A family. |
Aliases | CASP 1 antibody|CASP-1 antibody|CASP1 antibody|CASP1_HUMAN antibody|Caspase 1 apoptosis related cysteine peptidase antibody|Caspase 1 apoptosis related cysteine protease antibody|Caspase-1 subunit p10 antibody|Caspase1 antibody|ICE antibody|IL 1 beta converting enzyme antibody|IL 1BC antibody|IL-1 beta-converting enzyme antibody|IL-1BC antibody|IL1 beta converting enzyme antibody|IL1B convertase antibody|IL1BC antibody|IL1BCE antibody|Interleukin 1 beta convertase antibody|Interleukin 1 beta convertase precursor antibody|Interleukin 1 beta converting enzyme antibody|Interleukin 1B converting enzyme antibody|Interleukin-1 beta convertase antibody|Interleukin-1 beta-converting enzyme antibody|P45 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
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Anti-Caspase-1(P10) antibody, ASA-B0283, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
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Anti-Caspase-1(P10) antibody, ASA-B0283, Western blotting
All lanes: Anti CASP1(P10) (ASA-B0283) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 45KD
Observed bind size: 45KD
All lanes: Anti CASP1(P10) (ASA-B0283) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 45KD
Observed bind size: 45KD
-antibody-ASA-B0283-3.jpg)