More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| caspase 9, apoptosis-related cysteine peptidase | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Caspase 9(183-198aa SNIDCEKLRRRFSSLH). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CASP9 |
Protein | Caspase-9(CASP-9) |
Uniprot ID | P55211 |
Function | Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf- 1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP- ribose) polymerase (PARP). |
Tissue Specificity | Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes. |
Sub-cellular localization | |
Sequence Similarities | Belongs to the peptidase C14A family. |
Aliases | APAF 3 antibody|APAF-3 antibody|APAF3 antibody|Apoptosis related cysteine peptidase antibody|Apoptotic protease activating factor 3 antibody|Apoptotic protease Mch 6 antibody|Apoptotic protease Mch-6 antibody|Apoptotic protease-activating factor 3 antibody|CASP 9 antibody|CASP-9 antibody|CASP9 antibody|CASP9_HUMAN antibody|Caspase 9 apoptosis related cysteine peptidase antibody|Caspase 9 Dominant Negative antibody|Caspase 9c antibody|Caspase-9 antibody|Caspase-9 subunit p10 antibody|Caspase9 antibody|ICE LAP6 antibody|ICE like apoptotic protease 6 antibody|ICE-LAP6 antibody|ICE-like apoptotic protease 6 antibody|MCH6 antibody|RNCASP9 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Caspase 9 antibody, ASA-B0306, Western blotting
All lanes: Anti Caspase 9 (ASA-B0306) at 0.5ug/ml
Lane 1: SMMC Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: CEM Whole Cell Lysate at 40ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Lane 5: RAJI Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Predicted bind size: 35KD
Observed bind size: 35KD
All lanes: Anti Caspase 9 (ASA-B0306) at 0.5ug/ml
Lane 1: SMMC Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: CEM Whole Cell Lysate at 40ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Lane 5: RAJI Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Predicted bind size: 35KD
Observed bind size: 35KD
Anti-Caspase 9 antibody, ASA-B0306, IHC(P)
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue
