cardiac Troponin C Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
troponin C type 1(slow)	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human cardiac Troponin C(1-18aa MDDIYKAAVEQLTEEQKN), identical to the related rat and mouse sequences .

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	TNNC1
Protein	Troponin C, slow skeletal and cardiac muscles
Uniprot ID	P63316
Function	Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments.
Tissue Specificity
Sub-cellular localization
Sequence Similarities	Belongs to the troponin C family.
Aliases	tnnc1a antibody|Cardiac troponin C antibody|CMH7 antibody|cTnI antibody|slow skeletal and cardiac muscles antibody|Slow troponin C1 antibody|TN C antibody|TN-C antibody|TNC antibody|TNNC 1 antibody|TNNC antibody|TNNC1 antibody|TNNC1 protein antibody|TNNC1_HUMAN antibody|TNNI3 antibody|Troponin C antibody|Troponin C cardiac antibody|Troponin C slow antibody|Troponin C slow skeletal and cardiac muscles antibody|Troponin C type 1(slow) antibody|Troponin C type 1 antibody|Troponin C1 slow antibody|Troponin I type 3(cardiac) antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Rat Human, Mouse	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human, Rat Mouse	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information