More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| troponin C type 1(slow) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human cardiac Troponin C(1-18aa MDDIYKAAVEQLTEEQKN), identical to the related rat and mouse sequences . | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TNNC1 |
Protein | Troponin C, slow skeletal and cardiac muscles |
Uniprot ID | P63316 |
Function | Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments. |
Tissue Specificity | |
Sub-cellular localization | |
Sequence Similarities | Belongs to the troponin C family. |
Aliases | tnnc1a antibody|Cardiac troponin C antibody|CMH7 antibody|cTnI antibody|slow skeletal and cardiac muscles antibody|Slow troponin C1 antibody|TN C antibody|TN-C antibody|TNC antibody|TNNC 1 antibody|TNNC antibody|TNNC1 antibody|TNNC1 protein antibody|TNNC1_HUMAN antibody|TNNI3 antibody|Troponin C antibody|Troponin C cardiac antibody|Troponin C slow antibody|Troponin C slow skeletal and cardiac muscles antibody|Troponin C type 1(slow) antibody|Troponin C type 1 antibody|Troponin C1 slow antibody|Troponin I type 3(cardiac) antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Human, Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-cardiac Troponin C antibody, ASA-B0282, Western blotting
All lanes: Anti cardiac Troponin C (ASA-B0282) at 0.5ug/ml
WB: Rat Skeletal Muscle Tissue Lysate at 50ug
Predicted bind size: 18KD
Observed bind size: 18KD
All lanes: Anti cardiac Troponin C (ASA-B0282) at 0.5ug/ml
WB: Rat Skeletal Muscle Tissue Lysate at 50ug
Predicted bind size: 18KD
Observed bind size: 18KD
Anti-cardiac Troponin C antibody, ASA-B0282, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
IHC(P): Rat Cardiac Muscle Tissue
