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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| nucleotide-binding oligomerization domain containing 1 | Polyclonal | IgG | Rabbit | Human, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human CARD4 recombinant protein (Position: M1-M160). Human CARD4 shares 82% amino acid (aa) sequence identity with mouse CARD4. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | NOD1 |
Protein | Nucleotide-binding oligomerization domain-containing protein 1 |
Uniprot ID | Q9Y239 |
Function | Enhances caspase-9-mediated apoptosis. Induces NF-kappa- B activity via RIPK2 and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Forms an intracellular sensing system along with ARHGEF2 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIPK2 dependent NF-kappa-B signaling pathway activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides but also in the activation of NF-kappa-B by Shigella effector proteins IpgB2 and OspB. Recruits NLRP10 to the cell membrane following bacterial infection. |
Tissue Specificity | Highly expressed in adult heart, skeletal muscle, pancreas, spleen and ovary. Also detected in placenta, lung, liver, kidney, thymus, testis, small intestine and colon. |
Sub-cellular localization | Cytoplasm. Cell membrane. Apical cell membrane. Basolateral cell membrane. Note: Detected in the cytoplasm and at the cell membrane. Following bacterial infection, localizes to bacterial entry sites in the cell membrane. Recruited to the basolateral and apical membranes in polarized epithelial cells. |
Sequence Similarities | Contains 1 CARD domain. |
Aliases | CARD 4 antibody|CARD4 antibody|Caspase recruitment domain 4 antibody|Caspase recruitment domain containing protein 4 antibody|Caspase recruitment domain family member 4 antibody|Caspase recruitment domain protein 4 antibody|Caspase recruitment domain-containing protein 4 antibody|CLR 7.1 antibody|CLR7.1 antibody|NLR family CARD domain containing 1 antibody|NLRC 1 antibody|NLRC1 antibody|NOD 1 antibody|Nod1 antibody|NOD1 protein antibody|NOD1_HUMAN antibody|Nucleotide binding oligomerization domain containing 1 antibody|Nucleotide binding oligomerization domain leucine rich repeat and CARD domain containing 1 antibody|Nucleotide-binding oligomerization domain-containing protein 1 antibody|Protein Nod1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- CARD4 antibody, ASA-B0280, Western blotting
All lanes: Anti CARD4 (ASA-B0280) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: Rat Cardiac Muscle Tissue Lysate at 50ug
Predicted bind size: 107KD
Observed bind size: 107KD
All lanes: Anti CARD4 (ASA-B0280) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: Rat Cardiac Muscle Tissue Lysate at 50ug
Predicted bind size: 107KD
Observed bind size: 107KD
Anti- CARD4 antibody, ASA-B0280,IHC(P)
IHC(P): Human Placenta Tissue
IHC(P): Human Placenta Tissue
