More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| calbindin 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminal of human Calretinin(234-262aa EMNIQQLTNYRKSVMSLAEAGKLYRKDLE), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CALB2 |
Protein | Calretinin |
Uniprot ID | P22676 |
Function | Calretinin is a calcium-binding protein which is abundant in auditory neurons. |
Tissue Specificity | Brain. |
Sub-cellular localization | |
Sequence Similarities | Belongs to the calbindin family. |
Aliases | 29 kDa calbindin antibody|CAB 29 antibody|CAB29 antibody|CAL 2 antibody|CAL2 antibody|CALB 2 antibody|CALB2 antibody|CALB2_HUMAN antibody|Calbindin 2 29kDa antibody|Calbindin 2 antibody|Calbindin D29K antibody|Calbindin2 antibody|Calretinin antibody|CR antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Calretinin antibody, ASA-B0274, Western blotting
All lanes: Anti Calretinin (ASA-B0274) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 31KD
Observed bind size: 38KD
All lanes: Anti Calretinin (ASA-B0274) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 31KD
Observed bind size: 38KD
Anti- Calretinin antibody, ASA-B0274, IHC(P)
IHC(P): Mouse Brain Tissue
IHC(P): Mouse Brain Tissue
Anti- Calretinin antibody, ASA-B0274, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue