More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| calponin 2 | Monoclonal | IgG | Mouse | Chicken, Human, Turkey | IHC-P, ICC, WB | Ascites |
Immunogen |
| Turkey gizzard calponin |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CNN2 |
Protein | Calponin-2 |
Uniprot ID | Human(Q99439), Mouse(Q08093) |
Function | Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. |
Tissue Specificity | Heart and smooth muscle. |
Sub-cellular localization | |
Sequence Similarities | Belongs to the calponin family. |
Aliases | Calponin H2, smooth muscle|Neutral calponin |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.25-0.5μg/ml | Human, chicken, turkey | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, chicken, turkey | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | Suitable | Human, chicken, turkey | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
