More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| calponin 1, basic, smooth muscle | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Calponin(283-297aa EPAHNHHAHNYYNSA), different from the related mouse and rat sequences by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CNN1 |
Protein | Calponin-1 |
Uniprot ID | P51911 |
Function | Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity). |
Tissue Specificity | Smooth muscle, and tissues containing significant amounts of smooth muscle. |
Sub-cellular localization | |
Sequence Similarities | Belongs to the calponin family. |
Aliases | Basic calponin antibody|Calponin 1 antibody|Calponin 1 basic smooth muscle antibody|Calponin H1 antibody|Calponin H1 smooth muscle antibody|Calponin-1 antibody|Calponin1 antibody|Calponins basic antibody|CNN 1 antibody|Cnn1 antibody|CNN1_HUMAN antibody|Sm Calp antibody|SMCC antibody|smooth muscle antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Calponin antibody, ASA-B0272, IHC(P)
IHC(P): Human Placenta Tissue
IHC(P): Human Placenta Tissue
Anti-Calponin antibody, ASA-B0272,Western blotting
All lanes: Anti -Calponin (ASA-B0272) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 33KD
All lanes: Anti -Calponin (ASA-B0272) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 33KD
