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Calpain 1 Antibody

ASA-B0269

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
calpain 1,(mu/I) large subunit Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, IHC-F, ICC, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Calpain 1(312-326aa EWNNVDPYERDQLRV), different from the mouse sequence by two amino acids.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 CAPN1

Protein

 Calpain-1 catalytic subunit

Uniprot ID

 P07384

Function

 Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Tissue Specificity

Ubiquitous.

Sub-cellular localization

 Cytoplasm.

Sequence Similarities

 Belongs to the peptidase C2 family.

Aliases

 Ca2 activated neutral protease antibody|Calcium activated neutral proteinase 1 antibody|Calcium activated neutral proteinase antibody|Calcium activated neutral proteinase small subunit antibody|Calcium dependent protease small subunit 1 antibody|Calcium dependent protease small subunit antibody|Calcium-activated neutral proteinase 1 antibody|Calpain 1(mu/I) large subunit antibody|Calpain 1 antibody|Calpain 1 catalytic subunit antibody|Calpain 1 large subunit antibody|Calpain Large Polypeptide L1 antibody|Calpain Large Polypeptide L1 antibody|Calpain mu type antibody|Calpain mu-type antibody|Calpain regulatory subunit antibody|Calpain small subunit 1 antibody|Calpain-1 catalytic subunit antibody|Calpain-1 large subunit antibody|Calpain1 antibody|CAN1_HUMAN antibody|CANP 1 antibody|CANP antibody|CANP small subunit antibody|CANPL 1 antibody|CANPL1 antibody|CAPN 1 antibody|CAPN1 antibody|Cell proliferation inducing protein 30 antibody|Cell proliferation-inducing gene 30 protein antibody|Micromolar Calpain antibody|Micromolar-calpain antibody|Mu Calpain antibody|muCANP antibody|muCL antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Mouse, RatAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, RatAssaySolutio's IHC/ICC Detection kit
Immunohistochemistry(Frozen Section) 0.5-1μg/ml Rat Human, MouseAssaySolutio's IHC/ICC Detection kit
Immunocytochemistry 0.5-1μg/ml Human Human, MouseAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Calpain 1 antibody, ASA-B0269, Western blotting
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Anti-Calpain 1 antibody, ASA-B0269, IHC(P)
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Anti-Calpain 1 antibody, ASA-B0269, ICC
ICC: HELA Cell

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