More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| calpain 1, (mu/I) large subunit | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Calpain 1 recombinant protein (Position: Q396-A555). Human Calpain 1 shares 86% amino acid (aa) sequence identity with both mouse and rat Calpain 1. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CAPN1 |
Protein | Calpain-1 catalytic subunit |
Uniprot ID | P07384 |
Function | Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. |
Tissue Specificity | Ubiquitous. |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | Belongs to the peptidase C2 family. |
Aliases | Ca2 activated neutral protease antibody|Calcium activated neutral proteinase 1 antibody|Calcium activated neutral proteinase antibody|Calcium activated neutral proteinase small subunit antibody|Calcium dependent protease small subunit 1 antibody|Calcium dependent protease small subunit antibody|Calcium-activated neutral proteinase 1 antibody|Calpain 1 (mu/I) large subunit antibody|Calpain 1 antibody|Calpain 1 catalytic subunit antibody|Calpain 1 large subunit antibody|Calpain 1, (mu/I) large subunit antibody|Calpain Large Polypeptide L1 antibody|Calpain mu type antibody|Calpain mu-type antibody|Calpain regulatory subunit antibody|Calpain small subunit 1 antibody|Calpain, large polypeptide L1 antibody|Calpain-1 catalytic subunit antibody|Calpain-1 large subunit antibody|Calpain1 antibody|CAN1_HUMAN antibody|CANP 1 antibody|CANP antibody|CANP small subunit antibody|CANP1 antibody|CANPL 1 antibody|CANPL1 antibody|CAPN 1 antibody|CAPN1 antibody|Cell proliferation inducing protein 30 antibody|Cell proliferation-inducing gene 30 protein antibody|Micromolar Calpain antibody|Micromolar-calpain antibody|Mu Calpain antibody|muCANP antibody|muCL antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Calpain1 antibody, ASA-B0270, Western blotting
All lanes: Anti Calpain1 (ASA-B0270) at 0.5ug/ml
Lane 1: Rat Lung Tissue Lysate at 50ug
Lane 2: Mouse Lung Tissue Lysate at 50ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 82KD
Observed bind size: 82KD
All lanes: Anti Calpain1 (ASA-B0270) at 0.5ug/ml
Lane 1: Rat Lung Tissue Lysate at 50ug
Lane 2: Mouse Lung Tissue Lysate at 50ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 82KD
Observed bind size: 82KD
Anti- Calpain1 antibody, ASA-B0270, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
