More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| caldesmon 1 | Monoclonal | IgG | Mouse | Human, Mouse, Rabbit | IHC-P, WB | Ascites |
Immunogen | ||||||
| Human uterus smooth muscle extract. | ||||||
Properties
Form | Liquid |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | |
Storage | At -20 °C for 12 months, as supplied, at 2-8 °C for one month. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CALD1 |
Protein | Caldesmon |
Uniprot ID | Human(Q05682), Mouse(Q8VCQ8), Rat(Q62736) |
Function | Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity). |
Tissue Specificity | High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular- weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart. |
Sub-cellular localization | Cytoplasm, cytoskeleton . Cytoplasm, myofibril . Note: On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle). |
Sequence Similarities | |
Aliases | CDM|H-CAD|L-CAD |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.25-0.5μg/ml | Human, mouse, rabbit | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, mouse, rabbit | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
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Anti-Caldesmon(smooth) antibody, ASA-B0266, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
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Anti-Caldesmon(smooth) antibody, ASA-B0266, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
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