More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| calbindin 1, 28kDa | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Calbindin recombinant protein (Position: A2-E175). Human Calbindin shares 99% amino acid (aa) sequence identity with both mouse and rat Calbindin. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CALB1 |
Protein | Calbindin |
Uniprot ID | P05937 |
Function | Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. |
Tissue Specificity | |
Sub-cellular localization | |
Sequence Similarities | Belongs to the calbindin family. |
Aliases | avian-type antibody|CAB27 antibody|CALB 1 antibody|CALB antibody|CALB1 antibody|CALB1_HUMAN antibody|Calbindin 1 28kDa antibody|Calbindin antibody|Calbindin D28 antibody|D 28K antibody|D-28K antibody|D28K antibody|OTTHUMP00000166027 antibody|OTTHUMP00000225441 antibody|RTVL H protein antibody|Vitamin D dependent calcium binding protein antibody|Vitamin D dependent calcium binding protein avian type antibody|Vitamin D dependent calcium binding protein avian-type antibody|Vitamin D-dependent calcium-binding protein antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Calbindin antibody, ASA-B0262--1.jpg
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
Anti-Calbindin antibody, ASA-B0262--2.jpg
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
Anti-Calbindin antibody, ASA-B0262--3.jpg
IHC(P): Mouse Kidney Tissue
IHC(P): Mouse Kidney Tissue