More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| complement component 5 | Polyclonal | IgG | Rabbit | Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived rat C5a recombinant protein (Position: D1-R77). Rat C5a shares 62% and 82% amino acid (aa) sequences identity with human and mouse C5a, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | C5 |
Protein | Complement C5 |
Uniprot ID | P08650 |
Function | Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation. |
Tissue Specificity | |
Sub-cellular localization | Secreted. |
Sequence Similarities | |
Aliases | Anaphylatoxin C5a analog antibody|Anaphylatoxin C5a antibody|C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4 antibody|C5 antibody|C5a antibody|CO5_HUMAN antibody|Complement C5 alpha'' chain antibody|Complement C5 antibody|Complement component C5 antibody|Complement component C5a antibody|CPAMD 4 antibody|CPAMD4 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- C5A antibody, ASA-B0257, Western blotting
All lanes: Anti C5A (ASA-B0257) at 0.5ug/ml
WB: Recombinant Rat C5A Protein 0.5ng
Predicted bind size: 37KD
Observed bind size: 37KD
All lanes: Anti C5A (ASA-B0257) at 0.5ug/ml
WB: Recombinant Rat C5A Protein 0.5ng
Predicted bind size: 37KD
Observed bind size: 37KD
Anti- C5A antibody, ASA-B0257, Western blotting
All lanes: Anti C5A (ASA-B0257) at 0.5ug/ml
WB: Rat Liver Tissue Lysate at 50ug
Predicted bind size: 115KD
Observed bind size: 115KD
All lanes: Anti C5A (ASA-B0257) at 0.5ug/ml
WB: Rat Liver Tissue Lysate at 50ug
Predicted bind size: 115KD
Observed bind size: 115KD
Anti- C5A antibody, ASA-B0257, IHC(P)
IHC(P): Rat Spleen Tissue
IHC(P): Rat Spleen Tissue