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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| breast cancer 1, early onset | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human BRCA1 recombinant protein (Position: E1661-Y1863). Human BRCA1 shares 65% and 66% amino acid (aa) sequences identity with mouse and rat BRCA1, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | BRCA1 |
Protein | Breast cancer type 1 susceptibility protein |
Uniprot ID | P38398 |
Function | E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for normal cell cycle progression from G2 to mitosis. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks. Component of the BRCA1-RBBP8 complex which regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage via BRCA1-mediated ubiquitination of RBBP8. Acts as a transcriptional activator (PubMed:20160719). |
Tissue Specificity | Isoform 1 and isoform 3 are widely expressed. Isoform 3 is reduced or absent in several breast and ovarian cancer cell lines. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Contains 2 BRCT domains. |
Aliases | BRAC 1 antibody|BRCA 1 antibody|BRCA1 antibody|BRCA1/BRCA2 containing complex subunit 1 antibody|BRCA1/BRCA2-containing complex, subunit 1 antibody|BRCA1_HUMAN antibody|BRCAI antibody|BRCC 1 antibody|BRCC1 antibody|Breast and ovarian cancer susceptibility protein 1 antibody|Breast Cancer 1 antibody|Breast Cancer 1 Early Onset antibody|Breast cancer type 1 susceptibility protein antibody|Breast Ovarian Cancer Susceptibility antibody|Breast Ovarian Cancer Susceptibility antibody|BROVCA1 antibody|IRIS antibody|Papillary Serous Carcinoma Of The Peritoneum antibody|PNCA4 antibody|PPP1R53 antibody|Protein phosphatase 1 regulatory subunit 53 antibody|PSCP antibody|RING finger protein 53 antibody|RING finger protein 53 antibody|RNF53 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-BRCA1 antibody, ASA-B0243--1.jpg
IHC(P): Mouse Testis Tissue
IHC(P): Mouse Testis Tissue
Anti-BRCA1 antibody, ASA-B0243--2.jpg
IHC(P): Rat Testis Tissue
IHC(P): Rat Testis Tissue
Anti-BRCA1 antibody, ASA-B0243--3.jpg
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue