Bone Sialoprotein Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
integrin-binding sialoprotein	Polyclonal	IgG	Rabbit	Mouse, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Bone Sialoprotein(305-324aa SYYKGHGYEGYEGQNYYYHQ), different from the related rat sequence by one amino acid.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	IBSP
Protein	Bone sialoprotein 2
Uniprot ID	Q61711
Function	Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment (By similarity).
Tissue Specificity
Sub-cellular localization	Secreted.
Sequence Similarities
Aliases	BNSP antibody|Bone sialoprotein 2 antibody|Bone sialoprotein II antibody|BSP antibody|BSP II antibody|BSPII antibody|Cell binding sialoprotein antibody|Cell-binding sialoprotein antibody|IBSP antibody|Integrin binding sialoprotein antibody|Integrin-binding sialoprotein antibody|SIAL_HUMAN antibody|SPII antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Rat Mouse	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Mouse, Rat Mouse	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information