More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| integrin-binding sialoprotein | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
| A synthetic peptide corresponding to a sequence at the N-terminus of human Bone Sialoprotein(17-34aa FSMKNLHRRVKIEDSEEN), different from the related rat and mouse sequences by three amino acids. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | IBSP |
Protein | Bone sialoprotein 2 |
Uniprot ID | P21815 |
Function | Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment. |
Tissue Specificity | |
Sub-cellular localization | Secreted. |
Sequence Similarities | |
Aliases | BNSP antibody|Bone sialoprotein 2 antibody|Bone sialoprotein 2 precursor antibody|Bone sialoprotein II antibody|bone sialoprotein II; bone sialoprotein antibody|BSP antibody|BSP II antibody|BSPII antibody|Cell binding sialoprotein antibody|Cell-binding sialoprotein antibody|IBSP antibody|Integrin binding sialoprotein antibody|Integrin binding sialoprotein(bone sialoprotein II) antibody|integrin binding sialoprotein(bone sialoprotein, bone sialoprotein II) antibody|Integrin-binding sialoprotein antibody|SIAL_HUMAN antibody|SPII antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Bone Sialoprotein antibody, ASA-B0239, IHC(P)
IHC(P): Human Osteosarcoma Tissue
