More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| POU class 2 associating factor 1 | Polyclonal | IgG | Rabbit | Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of mouse BOB1(238-256aa EEEESNTYELNHTLSVEGF), different from the related rat sequence by one amino acid, and from human sequence by four amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | POU2AF1 |
Protein | POU domain class 2-associating factor 1 |
Uniprot ID | Q64693 |
Function | Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. |
Tissue Specificity | B-cell specific. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | |
Aliases | B cell Oct binding protein 1 antibody|B cell specific coactivator OBF 1 antibody|B cell specific coactivator OBF1 antibody|B-cell-specific coactivator OBF-1 antibody|BOB 1 antibody|BOB-1 antibody|OBF 1 antibody|OBF1 antibody|OBF1_HUMAN antibody|OCA B antibody|OCA-B antibody|OCAB antibody|OCT binding factor 1 antibody|OCT-binding factor 1 antibody|POU domain class 2 associating factor 1 antibody|POU domain class 2-associating factor 1 antibody|POU2AF1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-BOB1 antibody, ASA-B0237, Western blotting
Lane: Rat Spleen Tissue Lysate
Lane: Rat Spleen Tissue Lysate
Anti-BOB1 antibody, ASA-B0237, IHC(P)
IHC(P): Rat Tonsil Tissue
IHC(P): Rat Tonsil Tissue
