More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| bridging integrator 1 | Monoclonal | IgG | Mouse | Human, Mouse, Rat | IHC-F, ICC, WB | Ascites |
Immunogen | ||||||
| Recombinant polypeptide containing amino acids 189-398 of human Bin1. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | BIN1 |
Protein | Myc box-dependent-interacting protein 1 |
Uniprot ID | Human(O00499), Mouse(O08539), Rat(O08839) |
Function | May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation. |
Tissue Specificity | Ubiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier. Isoform BIN1 is widely expressed with highest expression in skeletal muscle. |
Sub-cellular localization | Isoform BIN1: Nucleus. |
Sequence Similarities | Contains 1 BAR domain. |
Aliases | AMPHL|AMPH2|SH3P9|amphiphysin II|Amphiphysin-like protein;Box-dependent myc-interacting protein 1;Bridging integrator 1 |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.25μg/ml | Human, mouse, rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Frozen Section) | 0.5μg/ml | Human, mouse, rat | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | Suitable | Human, mouse, rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
-ASA-B0231-1.jpg)
Anti-BIN1 antibody (monoclonal), ASA-B0231, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Skeletal Muscle Tissue Lysate
Lane 3: Rat Heart Tissue Lysate
Lane 4: Rat Kidney Tissue Lysate
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Skeletal Muscle Tissue Lysate
Lane 3: Rat Heart Tissue Lysate
Lane 4: Rat Kidney Tissue Lysate
-ASA-B0231-2.jpg)