BIN1 Antibody (monoclonal)

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
bridging integrator 1	Monoclonal	IgG	Mouse	Human, Mouse, Rat	IHC-F, ICC, WB	Ascites
Immunogen
Recombinant polypeptide containing amino acids 189-398 of human Bin1.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request.
Concentration	1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml.
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	BIN1
Protein	Myc box-dependent-interacting protein 1
Uniprot ID	Human(O00499), Mouse(O08539), Rat(O08839)
Function	May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation.
Tissue Specificity	Ubiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier. Isoform BIN1 is widely expressed with highest expression in skeletal muscle.
Sub-cellular localization	Isoform BIN1: Nucleus.
Sequence Similarities	Contains 1 BAR domain.
Aliases	AMPHL|AMPH2|SH3P9|amphiphysin II|Amphiphysin-like protein;Box-dependent myc-interacting protein 1;Bridging integrator 1

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.25μg/ml	Human, mouse, rat	AssaySolutio's ECL kit
Immunohistochemistry(Frozen Section)	0.5μg/ml	Human, mouse, rat	AssaySolutio's IHC/ICC Detection kit
Immunocytochemistry	Suitable	Human, mouse, rat	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information