Bid Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
BH3 interacting domain death agonist	Polyclonal	IgG	Rabbit	Mouse, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Bid(179-195aa NQNLFSYVRNLVRNEMD), different from the related rat sequence by one amino acid.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	Bid
Protein	BH3-interacting domain death agonist
Uniprot ID	P70444
Function	Induces caspases and apoptosis. Counters the protective effect of Bcl-2. The major proteolytic product p15 BID allows the release of cytochrome c.
Tissue Specificity
Sub-cellular localization	Cytoplasm.
Sequence Similarities
Aliases	Apoptic death agonist antibody|Apoptotic death agonist antibody|Apoptotic death agonist BID antibody|BH3 interacting domain death agonist antibody|BH3 interacting domain death agonist p11 antibody|BH3 interacting domain death agonist p13 antibody|BH3 interacting domain death agonist p15 antibody|BH3-interacting domain death agonist p11 antibody|BID antibody|BID isoform ES(1b) antibody|BID isoform L(2) antibody|BID isoform Si6 antibody|BID_HUMAN antibody|Desmocollin type 4 antibody|FP497 antibody|HGNC:1050 antibody|Human BID coding sequence antibody|MGC15319 antibody|MGC42355 antibody|p11 BID antibody|p13 BID antibody|p15 BID antibody|p22 BID antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Mouse Rat	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Rat Mouse	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information