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beta-Actin Antibody (monoclonal)

ASA-B0217

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
actin, beta Monoclonal IgG Mouse Human, Mouse, Rat IHC-P, WB Ascites

Immunogen

slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request.

Concentration

 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml.

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 ACTB

Protein

 Actin, cytoplasmic 1

Uniprot ID

 Human(P60709), Mouse(P60710), Rat(P60711)

Function

 Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Tissue Specificity

Sub-cellular localization

 Cytoplasm, cytoskeleton.

Sequence Similarities

 Belongs to the actin family.

Aliases

 BRWS1|PS1TP5BP1|BETA-ACTIN|ACTIN, CYTOPLASMIC, 1

Application Details

Application Concentration* Species Validated Using**
Western blot0.25-0.5μg/mlHuman, mouse, ratAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)0.5-1μg/mlHuman, mouse, ratAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-beta-Actin antibody (monoclonal), ASA-B0217, Western blotting
Lane 1: Rat Ovary Tissue Lysate
Lane 2: Rat Testis Tissue Lysate
Lane 3: Rat Cardiac Muscle Tissue Lysate
Lane 4: Rat Brain Tissue Lysate
Lane 5: A453 Cell Lysate
Lane 6: HE
Anti-beta-Actin antibody (monoclonal), ASA-B0217, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
Lane 3: Rat Brain Tissue Lysate
Lane 4: Rat Kidney Tissue Lysate
Lane 5: HELA Cell Lysate
Lane 6: SMMC Cell
Anti-beta-Actin antibody (monoclonal), ASA-B0217, IHC(P)
IHC(P): Mouse Intestine Tissue

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