More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
defensin, beta 1 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
A synthetic peptide corresponding to a sequence at the C-terminus of human beta Defensin 1(52-68aa FTKIQGTCYRGKAKCCK). |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | DEFB1 |
Protein | Beta-defensin 1(BD-1/hBD-1) |
Uniprot ID | P60022 |
Function | Has bactericidal activity. |
Tissue Specificity | Plasma. |
Sub-cellular localization | Secreted. |
Sequence Similarities | Belongs to the beta-defensin family. |
Aliases | BD 1 antibody|BD-1 antibody|BD1 antibody|beta 1 antibody|Beta defensin 1 antibody|Beta-defensin 1 antibody|DEFB 1 antibody|DEFB1 antibody|DEFB1_HUMAN antibody|DEFB101 antibody|Defensin antibody|Defensin beta 1 antibody|Defensin beta 1 preproprotein antibody|HBD 1 antibody|hBD-1 antibody|HBD1 antibody|MGC51822 antibody |
Application Details
Application | Concentration* | Species | Validated Using** |
Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-beta Defensin 1 antibody, ASA-B0225, IHC(P)
IHC(P): Lung Cancer Tissue
Anti-beta Defensin 1 antibody, ASA-B0225,Western blotting
All lanes: Anti Defensin 1 (ASA-B0225) at 0.5ug/ml
WB : COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 7KD
Observed bind size: 7KD