More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| beclin 1, autophagy related | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Beclin 1 recombinant protein (Position: M1-S354). Human Beclin 1 shares 97% amino acid (aa) sequence identity with both mouse and rat Beclin 1. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | BECN1 |
Protein | Beclin-1 |
Uniprot ID | Q14457 |
Function | Plays a central role in autophagy (PubMed:23184933). Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3- C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). Protects against infection by a neurovirulent strain of Sindbis virus (PubMed:9765397). May play a role in antiviral host defense. |
Tissue Specificity | Ubiquitous. |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | |
Aliases | APG6 antibody|ATG 6 antibody|ATG6 antibody|ATG6 autophagy related 6 homolog antibody|Bcl-2-interacting protein beclin antibody|Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody|Beclin 1 autophagy related antibody|Beclin-1 antibody|Beclin1 antibody|BECN 1 antibody|BECN1 antibody|BECN1_HUMAN antibody|Coiled coil myosin like BCL2 interacting protein antibody|Coiled-coil myosin-like BCL2-interacting protein antibody|GT197 antibody|Protein GT197 antibody|VPS 30 antibody|VPS30 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Mouse, Rat Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Beclin 1 antibody, ASA-B0216--1.jpg
IHC(P): Mouse Intestine Tissue
IHC(P): Mouse Intestine Tissue
Anti-Beclin 1 antibody, ASA-B0216--2.jpg
IHC(P): Mouse Spleen Tissue
IHC(P): Mouse Spleen Tissue
Anti-Beclin 1 antibody, ASA-B0216--3.jpg
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue