More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| brain-derived neurotrophic factor | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human BDNF recombinant protein (Position: H129-R247). Human BDNF shares 100% amino acid (aa) sequence identity with both mouse and rat BDNF. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | BDNF |
Protein | Brain-derived neurotrophic factor |
Uniprot ID | P21237 |
Function | During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. |
Tissue Specificity | |
Sub-cellular localization | Secreted. |
Sequence Similarities | |
Aliases | Abrineurin antibody|BDNF antibody|BDNF_HUMAN antibody|Brain Derived Neurotrophic Factor antibody|Brain-derived neurotrophic factor antibody|MGC34632 antibody|Neurotrophin antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat, Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Mouse, Rat, Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-BDNF antibody, ASA-B0214, IHC(P)
Rat Brain Tissue
Anti-BDNF antibody, ASA-B0214, IHC(P)
Mouse Brain Tissue
Anti-BDNF antibody, ASA-B0214 Western blot
Anti-BDNF(ASA-B0214) at 0.5ug/ml
Rat Brain Tissue Lysate at 40ug
Predicted band size: 28KD
Observed band size: 28KD
Anti-BDNF(ASA-B0214) at 0.5ug/ml
Rat Brain Tissue Lysate at 40ug
Predicted band size: 28KD
Observed band size: 28KD