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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| BCL2-like 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Bcl-XS (120-130aa, YQSFEQDTFVE), different from the related rat and mouse sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | BCL2L1 |
Protein | Bcl-2-like protein 1(Bcl2-L-1) |
Uniprot ID | Q07817 |
Function | Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage- dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis. |
Tissue Specificity | Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain. |
Sub-cellular localization | Isoform Bcl-X(L): Mitochondrion inner membrane . Mitochondrion outer membrane . Mitochondrion matrix . Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane . Cytoplasm, cytosol . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus membrane ; Single-pass membrane protein ; Cytoplasmic side . Note: After neuronal stimulation, translocates from cytosol to synaptic vesicle and mitochondrion membrane in a calmodulin-dependent manner (By similarity). Localizes to the centrosome when phosphorylated at Ser-49. |
Sequence Similarities | Belongs to the Bcl-2 family. |
Aliases | Apoptosis regulator Bcl X antibody|Apoptosis regulator Bcl-X antibody|Apoptosis regulator BclX antibody|B cell lymphoma 2 like antibody|B2CL1_HUMAN antibody|Bcl 2 like 1 protein antibody|Bcl X antibody|Bcl xL antibody|BCL XL/S antibody|Bcl xS antibody|Bcl-2-like protein 1 antibody|Bcl2 Like 1 antibody|Bcl2 related gene antibody|Bcl2-L-1 antibody|Bcl2l1 antibody|BCLX antibody|BclXL antibody|BclXs antibody|DKFZp781P2092 antibody|PPP1R52 antibody|Protein phosphatase 1 regulatory subunit 52 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat, Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Bcl-XS antibody, ASA-B0204, Western blotting
All lanes: Anti Bcl-XS (ASA-B0204) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: PC-12 Whole Cell Lysate at 40ug
Predicted band size: 19KD
Observed band size: 19KD
All lanes: Anti Bcl-XS (ASA-B0204) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: PC-12 Whole Cell Lysate at 40ug
Predicted band size: 19KD
Observed band size: 19KD
Anti-Bcl-XS antibody, ASA-B0204, IHC(P)
Human Colon Cancer Tissue
Human Colon Cancer Tissue