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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| B-cell CLL/lymphoma 3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Bcl3 (199-216aa, MALDRHGQTAAHLACEHR), different from the related rat and mouse sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | 1884 |
Protein | B-cell lymphoma 3 protein(BCL-3) |
Uniprot ID | P20749 |
Function | Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation (By similarity). |
Tissue Specificity | |
Sub-cellular localization | Nucleus. Cytoplasm . Cytoplasm, perinuclear region . Note: Ubiquitination via 'Lys- 63'-linked ubiquitin chains is required for nuclear accumulation. |
Sequence Similarities | Contains 7 ANK repeats. |
Aliases | B cell CLL/lymphoma 3 antibody|B cell leukemia/lymphoma 3 antibody|B cell lymphoma 3 encoded protein antibody|B-cell lymphoma 3 protein antibody|Bcl 3 antibody|BCL 4 antibody|BCL-3 antibody|Bcl3 antibody|BCL3_HUMAN antibody|BCL4 antibody|Chronic lymphatic leukemia protein antibody|D19S37 antibody|Protein Bcl 3 antibody|Proto-oncogene BCL3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat, Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat, Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Bcl3 antibody, ASA-B0209, Western blotting
Lane 1: JURKAT Cell Lysate
Lane 2: MM231 Cell Lysate
Lane 3: RAJI Cell Lysate
Lane 4: CEM Cell Lysate
Lane 1: JURKAT Cell Lysate
Lane 2: MM231 Cell Lysate
Lane 3: RAJI Cell Lysate
Lane 4: CEM Cell Lysate
Anti-Bcl3 antibody, ASA-B0209, IHC(P)
Human Lung Cancer Tissue
Human Lung Cancer Tissue