More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| BCL2-like 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human BCL2L2 (74-88aa, SAQQRFTQVSDELFQ), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | BCL2L2 |
Protein | Bcl-2-like protein 2(Bcl2-L-2) |
Uniprot ID | Q92843 |
Function | Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX. |
Tissue Specificity | Expressed (at protein level) in a wide range of tissues with highest levels in brain, spinal cord, testis, pancreas, heart, spleen and mammary glands. Moderate levels found in thymus, ovary and small intestine. Not detected in salivary gland, muscle or liver. Also expressed in cell lines of myeloid, fibroblast and epithelial origin. Not detected in most lymphoid cell lines. |
Sub-cellular localization | Mitochondrion membrane. |
Sequence Similarities | Belongs to the Bcl-2 family. |
Aliases | Apoptosis regulator Bcl-W |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat, Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat, Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- BCL2L2 antibody, ASA-B0208, Western blotting
Anti BCL2L2 (ASA-B0208) at 0.5ug/ml
HELAWhole Cell Lysate at 40ug
Predicted band size: 21KD
Observed band size: 21KD
Anti BCL2L2 (ASA-B0208) at 0.5ug/ml
HELAWhole Cell Lysate at 40ug
Predicted band size: 21KD
Observed band size: 21KD
Anti- BCL2L2 antibody, ASA-B0208,IHC(P)
Human Rectal Cancer Tissue
Human Rectal Cancer Tissue