More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| B-cell CLL/lymphoma 2 | Monoclonal | IgG | Mouse | Human | IHC-P, IHC-F, ICC, WB | Ascites |
Immunogen | ||||||
| Synthetic peptide corresponding to residues 41-54 of the bcl-2 protein, conjugated to thyroglobulin. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | BCL2 |
Protein | Apoptosis regulator Bcl-2 |
Uniprot ID | Human(P10415), Mouse(P10417), Rat(P49950) |
Function | Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). |
Tissue Specificity | Expressed in a variety of tissues. |
Sub-cellular localization | Mitochondrion outer membrane. |
Sequence Similarities | Belongs to the Bcl-2 family. |
Aliases | BCL2|PPP1R50|protein phosphatase 1, regulatory subunit 50|ONCOGENE B-CELL LEUKEMIA 2 |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 1-2μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.4-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.4-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | Suitable | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
-ASA-B0203-1.jpg)
Anti-Bcl-2 antibody (monoclonal), ASA-B0203, Western blotting
Lane 1: Rat Heart Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
Lane 3: Rat Small Intestine Tissue Lysate
Lane 4: Rat Liver Tissue Lysate
Lane 1: Rat Heart Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
Lane 3: Rat Small Intestine Tissue Lysate
Lane 4: Rat Liver Tissue Lysate
-ASA-B0203-2.jpg)
Anti-Bcl-2 antibody (monoclonal), ASA-B0203, IHC(P)
Human Intestinal Cancer Tissue
Human Intestinal Cancer Tissue
-ASA-B0203-3.jpg)
Anti-Bcl-2 antibody (monoclonal), ASA-B0203, IHC(P)
Human Mammary Cancer Tissue
Human Mammary Cancer Tissue