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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| branched chain amino-acid transaminase 1, cytosolic | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human BCAT1 (360-375aa, KLASRILSKLTDIQYGR), different from the related mouse sequence by one amino acid, rat sequence by three amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | BCAT1 |
Protein | Branched-chain-amino-acid aminotransferase, cytosolic(BCAT(c)) |
Uniprot ID | P54687 |
Function | Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. |
Tissue Specificity | During embryogenesis, expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma. |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | |
Aliases | BCAT antibody|BCAT(c) antibody|Bcat1 antibody|BCAT1_HUMAN antibody|BCT1 antibody|Branched chain amino acid aminotransferase, cytosolic antibody|branched chain amino-acid transaminase 1, cytosolic antibody|branched chain aminotransferase 1 antibody|branched chain aminotransferase 1, cytosolic antibody|Branched-chain-amino-acid aminotransferase antibody|cytosolic antibody|DKFZp686E12175 antibody|EC 2.6.1.42 antibody|ECA39 antibody|MECA39 antibody|placental protein 18 antibody|PNAS 121 antibody|PP18 antibody|Protein ECA39 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-BCAT1 antibody, ASA-B0200, Western blotting
All lanes: Anti BCAT1 (ASA-B0200) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Predicted band size: 45KD
Observed band size: 45KD
All lanes: Anti BCAT1 (ASA-B0200) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Predicted band size: 45KD
Observed band size: 45KD
Anti-BCAT1 antibody, ASA-B0200, IHC(P)
Human Mammary Cancer Tissue
Human Mammary Cancer Tissue