More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| chemokine(C-X-C motif) ligand 13 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human BCA1 (78-94aa, DPQAEWIQRMMEVLRKR). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CXCL13 |
Protein | C-X-C motif chemokine 13 |
Uniprot ID | O43927 |
Function | Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B- lymphocytes. Binds to BLR1/CXCR5. |
Tissue Specificity | Highest levels in liver, followed by spleen, lymph node, appendix and stomach. Low levels in salivary gland, mammary gland and fetal spleen. |
Sub-cellular localization | Secreted. |
Sequence Similarities | Belongs to the intercrine alpha (chemokine CxC) family. |
Aliases | ANGIE antibody|ANGIE2 antibody|B cell attracting chemokine 1 antibody|B cell-attracting chemokine 1 antibody|B lymphocyte chemoattractant antibody|B-cell chemoattractant antibody|B-cell-attracting chemokine 1 antibody|BCA-1 antibody|BLC antibody|BLR1L antibody|C-X-C motif chemokine 13 antibody|Chemokine(C-X-C motif) ligand 13 antibody|Chemokine(C-X-C motif) ligand 13(B-cell chemoattractant) antibody|Chemokine, CXC motif, ligand 13 antibody|CXC chemokine BLC antibody|CXCL13 antibody|CXL13_HUMAN antibody|SCYB13 antibody|Small inducible cytokine B subfamily(Cys-X-Cys motif), member 13(B-cell chemoattractant) antibody|Small inducible cytokine B13 antibody|Small inducible cytokine subfamily B, member 13 antibody|Small-inducible cytokine B13 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-BCA1 antibody, ASA-B0199, Western blotting
Lane 1: Recombinant Human CXCL13 Protein 10ng
Lane 2: Recombinant Human CXCL13 Protein 5ng
Lane 3: Recombinant Human CXCL13 Protein 2.5ng
Lane 1: Recombinant Human CXCL13 Protein 10ng
Lane 2: Recombinant Human CXCL13 Protein 5ng
Lane 3: Recombinant Human CXCL13 Protein 2.5ng
Anti-BCA1 antibody, ASA-B0199, IHC(P)
Human Lung Cancer Tissue
Human Lung Cancer Tissue