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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| BCL2-antagonist/killer 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human BAK recombinant protein (Position: A22-S211). Human BAK shares 78.3 % amino acid (aa) sequence identity with mouse BAK. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | BAK1 |
Protein | Bcl-2 homologous antagonist/killer |
Uniprot ID | Q16611 |
Function | In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti- apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis. |
Tissue Specificity | Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle. |
Sub-cellular localization | Mitochondrion membrane ; Single-pass membrane protein . |
Sequence Similarities | Belongs to the Bcl-2 family. |
Aliases | Apoptosis Regulator Bak antibody|BAK antibody|BAK like antibody|Bak NT antibody|BAK_HUMAN antibody|BAK1 antibody|Bcl 2 homologous antagonist/ killer antibody|Bcl 2 like 7 protein antibody|Bcl-2 homologous antagonist/killer antibody|Bcl-2-like protein 7 antibody|BCL2 antagonist/killer 1 antibody|Bcl2 like 7 Protein antibody|Bcl2-L-7 antibody|BCL2L7 antibody|CDN1 antibody|Cell death inhibitor 1 antibody|MGC117255 antibody|MGC3887 antibody| NBak antibody|Pro apoptotic protein BAK antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- BAK antibody, ASA-B0194, Western blotting
All lanes: Anti BAK (ASA-B0194) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 2: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted band size: 23KD
Observed band size: 23KD
All lanes: Anti BAK (ASA-B0194) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 2: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted band size: 23KD
Observed band size: 23KD
Anti- BAK antibody, ASA-B0194, IHC(P)
Mouse Skeletal Muscle Tissue
Anti- BAK antibody, ASA-B0194, IHC(P)
Rat Skeletal Muscle Tissue