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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| BCL2-associated athanogene 5 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human BAG5 (415-429aa, QGEEKCKAARKQAVR), different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | BAG5 |
Protein | BAG family molecular chaperone regulator 5 |
Uniprot ID | Q9UL15 |
Function | Inhibits both auto-ubiquitination of PARK2 and ubiquitination of target proteins by PARK2 (By similarity). May function as a nucleotide exchange factor for HSP/HSP70, promoting ADP release, and activating Hsp70-mediated refolding. |
Tissue Specificity | |
Sub-cellular localization | |
Sequence Similarities | Contains 5 BAG domains. |
Aliases | KIAA0873|Bcl-2-associated athanogene 5| |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat, Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat, Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-BAG5 antibody, ASA-B0192, Western blotting
Lane 1: Rat Thymus Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
Lane 3: Rat Testis Tissue Lysate
Lane 4: PANC Cell Lysate
Lane 1: Rat Thymus Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
Lane 3: Rat Testis Tissue Lysate
Lane 4: PANC Cell Lysate
Anti-BAG5 antibody, ASA-B0192, IHC(P)
Human Placenta Tissue
Human Placenta Tissue