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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| BCL2-associated athanogene 3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Bag3(491-506aa LEKLEQKAIDVPGQVQ), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | BAG3 |
Protein | BAG family molecular chaperone regulator 3 |
Uniprot ID | O95817 |
Function | Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity. |
Tissue Specificity | |
Sub-cellular localization | |
Sequence Similarities | Contains 1 BAG domain. |
Aliases | BAG 3 antibody|BAG family molecular chaperone regulator 3 antibody|BAG-3 antibody|Bag3 antibody|BAG3_HUMAN antibody|Bcl 2 binding protein antibody|Bcl-2-associated athanogene 3 antibody|Bcl-2-binding protein Bis antibody|BCL2 associated athanogene 3 antibody|BCL2 binding athanogene 3 antibody|BIS antibody|Docking protein CAIR 1 antibody|Docking protein CAIR-1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Bag3 antibody, ASA-B0191, Western blotting
Lane 1: MCF-7 Cell Lysate
Lane 2: JURKAT Cell Lysate
Lane 3: A549 Cell Lysate
Lane 4: HELA Cell Lysate
Lane 5: COLO320 Cell Lysate
Lane 1: MCF-7 Cell Lysate
Lane 2: JURKAT Cell Lysate
Lane 3: A549 Cell Lysate
Lane 4: HELA Cell Lysate
Lane 5: COLO320 Cell Lysate
Anti-Bag3 antibody, ASA-B0191, IHC(P)
Human Intestinal Cancer Tissue
Human Intestinal Cancer Tissue
Anti-Bag3 antibody, ASA-B0191, IHC(P)
Human Mammary Cancer Tissue
Human Mammary Cancer Tissue