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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| BCL2-associated athanogene | Polyclonal | IgG | Rabbit | Human, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Bag1(322-344aa DTVEQNICQETERLQSTNFALAE), different from the related mouse sequence by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | BAG1 |
Protein | BAG family molecular chaperone regulator 1(BAG-1) |
Uniprot ID | Q99933 |
Function | Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli. |
Tissue Specificity | Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines (at protein level). |
Sub-cellular localization | Isoform 1: Nucleus. Cytoplasm. Note: Isoform 1 localizes predominantly to the nucleus. |
Sequence Similarities | Contains 1 BAG domain. |
Aliases | Bcl-2-associated athanogene 1 |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat, Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Bag1 antibody, ASA-B0188, Western blotting
Lane 1: Recombinant Human BAG-1 Protein 10ng
Lane 2: Recombinant Human BAG-1 Protein 5ng
Lane 3: Recombinant Human BAG-1 Protein 2.5ng
Lane 4: MCF-7 Cell Lysate
Lane 1: Recombinant Human BAG-1 Protein 10ng
Lane 2: Recombinant Human BAG-1 Protein 5ng
Lane 3: Recombinant Human BAG-1 Protein 2.5ng
Lane 4: MCF-7 Cell Lysate
Anti-Bag1 antibody, ASA-B0188, IHC(P)
Rat Intestine Tissue
Rat Intestine Tissue