More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| v-myb myeloblastosis viral oncogene homolog(avian)-like 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human B MyB(1-17aa MSRRTRCEDLDELHYQD), identical to the related mouse sequence and different from the related rat sequence by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MYBL2 |
Protein | Myb-related protein B |
Uniprot ID | P10244 |
Function | Transcription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Contains 3 HTH myb-type DNA-binding domains. |
Aliases | B-Myb antibody|BMyB antibody|MGC15600 antibody|MYB L2 antibody|Myb related protein B antibody|Myb-like protein 2 antibody|Myb-related protein B antibody|MybB antibody|MYBB_HUMAN antibody|MYBL 2 antibody|MYBL2 antibody|v myb avian myeloblastosis viral oncogene homolog like 2 antibody|v myb myeloblastosis viral oncogene homolog(avian) like 2 antibody|v myb myeloblastosis viral oncogene homolog like 2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-B MyB antibody, ASA-B0182, Western blotting
E.coli derived -recombinant Human MYBL2 (162aa tag+ M1-L245), 46.8KD
Lane 1: Recombinant Human MYBL2 Protein 10ng
Lane 2: Recombinant Human MYBL2 Protein 5ng
Lane 3: Recombinant Human MYBL2 Protein 2.5ng
E.coli derived -recombinant Human MYBL2 (162aa tag+ M1-L245), 46.8KD
Lane 1: Recombinant Human MYBL2 Protein 10ng
Lane 2: Recombinant Human MYBL2 Protein 5ng
Lane 3: Recombinant Human MYBL2 Protein 2.5ng
Anti-B MyB antibody, ASA-B0182, IHC(P)
Rat Brain Tissue
Rat Brain Tissue
Anti-B MyB antibody, ASA-B0182, Western blotting
Lane 1: Rat Spleen Tissue Lysate
Lane 2: Rat Thymus Tissue Lysate
Lane 3: Rat Brain Tissue Lysate
Lane 4: HELA Cell Lysate
Lane 5: COLO320 Cell Lysate
Lane 6: MCF-7 Cell Lysate
Lane 1: Rat Spleen Tissue Lysate
Lane 2: Rat Thymus Tissue Lysate
Lane 3: Rat Brain Tissue Lysate
Lane 4: HELA Cell Lysate
Lane 5: COLO320 Cell Lysate
Lane 6: MCF-7 Cell Lysate