More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| ataxin 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human ATX2 (1283-1313aa, QSALQPIPVSTTAHFPYMTHP- SVQAHHQQQL), identical to the related mouse sequence. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ATXN2 |
Protein | Ataxin-2 |
Uniprot ID | Q99700 |
Function | Involved in EGFR trafficking, acting as negative regulator of endocytic EGFR internalization at the plasma membrane. |
Tissue Specificity | Expressed in the brain, heart, liver, skeletal muscle, pancreas and placenta. Isoform 1 is predominant in the brain and spinal cord. Isoform 4 is more abundant in the cerebellum. In the brain, broadly expressed in the amygdala, caudate nucleus, corpus callosum, hippocampus, hypothalamus, substantia nigra, subthalamic nucleus and thalamus. |
Sub-cellular localization | Cytoplasm . |
Sequence Similarities | |
Aliases | Ataxin 2 antibody|ATXN2 antibody|Olivopontocerebellar ataxia 2, autosomal dominant antibody|SCA2 antibody|Spinocerebellar ataxia type 2 protein antibody| TNRC13 antibody| Trinucleotide repeat containing gene 13 protein antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- ATX2 antibody, ASA-B0178, Western blotting
Anti ATX2 (ASA-B0178) at 0.5ug/ml
Mouse Liver Tissue Lysate at 50ug
Predicted band size: 140KD
Observed band size: 140KD
Anti ATX2 (ASA-B0178) at 0.5ug/ml
Mouse Liver Tissue Lysate at 50ug
Predicted band size: 140KD
Observed band size: 140KD
Anti- ATX2 antibody, ASA-B0178, IHC(P)
Mouse Brain Tissue
Mouse Brain Tissue
Anti- ATX2 antibody, ASA-B0178, IHC(P)
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Rat Brain Tissue
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