More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| ATPase, Ca++ transporting, ubiquitous | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human ATP2A3 (1-30aa, MEAAHLLPAADVLRHFSVTAEGG- LSPAQVT), different from the related mouse sequence by five amino acids, and from the related rat sequence by six amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ATP2A3 |
Protein | Sarcoplasmic/endoplasmic reticulum calcium ATPase 3 |
Uniprot ID | Q93084 |
Function | This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. |
Tissue Specificity | Found in most tissues. Most abundant in thymus, trachea, salivary gland, spleen, bone marrow, lymph node, peripheral leukocytes, pancreas and colon. Also detected in fetal tissues. Expressed in cell lineages of hematopoietic, epithelial, or embryonic origin and also expressed in several cancer cell lines. |
Sub-cellular localization | Nucleus membrane. |
Sequence Similarities | |
Aliases | Adenosine triphosphatase calcium antibody|AT2A3_HUMAN antibody|ATP2A3 antibody|ATPase Ca(2+) transporting ubiquitous antibody|ATPase Ca++ transporting ubiquitous antibody|Calcium pump 3 antibody|Calcium translocating P type ATPase antibody|Sarco/endoplasmic reticulum Ca2+ ATPase antibody|Sarcoplasmic/endoplasmic reticulum calcium ATPase 3 antibody|SERCA 3 antibody|SERCA3 antibody|SERCA3b antibody|SR Ca(2+) ATPase 3 antibody|SR Ca(2+)-ATPase 3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat, Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Mouse, Rat, Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- ATP2A3 antibody, ASA-B0173, Western blotting
All lanes: Anti ATP2A3 (ASA-B0173) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 2: Mouse Skeletal Muscle Tissue Lysate at 50ug
Predicted band size: 114KD
Observed band size: 114KD
All lanes: Anti ATP2A3 (ASA-B0173) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 2: Mouse Skeletal Muscle Tissue Lysate at 50ug
Predicted band size: 114KD
Observed band size: 114KD
Anti- ATP2A3 antibody, ASA-B0173, IHC(P)
Mouse Thymus Tissue
Mouse Thymus Tissue
Anti- ATP2A3 antibody, ASA-B0173,IHC(P)
Rat Thymus Tissue
Rat Thymus Tissue