More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human ATP2A2 (1-32aa, MENAHTKTVEEVLGHFGVNESTG- LSLEQVKKL), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | |
Protein | Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 |
Uniprot ID | P16615 |
Function | This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform 2 is involved in the regulation of the contraction/relaxation cycle. |
Tissue Specificity | Isoform 1 is widely expressed in smooth muscle and nonmuscle tissues such as in adult skin epidermis, with highest expression in liver, pancreas and lung, and intermediate expression in brain, kidney and placenta. Also expressed at lower levels in heart and skeletal muscle. Isoforms 2 and 3 are highly expressed in the heart and slow twitch skeletal muscle. Expression of isoform 3 is predominantly restricted to cardiomyocytes and in close proximity to the sarcolemma. Both isoforms are mildly expressed in lung, kidney, liver, pancreas and placenta. Expression of isoform 3 is amplified during monocytic differentiation and also observed in the fetal heart. |
Sub-cellular localization | Endoplasmic reticulum membrane; Multi-pass membrane protein. Sarcoplasmic reticulum membrane; Multi-pass membrane protein. |
Sequence Similarities | Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. |
Aliases | AT2A2_HUMAN antibody|ATP2A2 antibody|ATP2B antibody|ATPase Ca++ transporting cardiac muscle slow twitch 2 antibody|Calcium pump 2 antibody|Calcium-transporting ATPase sarcoplasmic reticulum type antibody|Calcium-transporting ATPase sarcoplasmic reticulum type slow twitch skeletal muscle isoform antibody|Cardiac Ca2+ ATPase antibody|DAR antibody|DD antibody|Endoplasmic reticulum class 1/2 Ca(2+) ATPase antibody|MGC45367 antibody|Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 antibody|SERCA 2 antibody|SERCA2 antibody|serca2a antibody|slow twitch skeletal muscle isoform antibody|SR Ca(2+)-ATPase 2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat, Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Mouse, Rat, Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- ATP2A2 antibody, ASA-B0172, Western blotting
All lanes: Anti ATP2A2 (ASA-B0172) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 2: Mouse Skeletal Muscle Tissue Lysate at 50ug
Predicted band size: 115KD
Observed band size: 115KD
All lanes: Anti ATP2A2 (ASA-B0172) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 2: Mouse Skeletal Muscle Tissue Lysate at 50ug
Predicted band size: 115KD
Observed band size: 115KD
Anti- ATP2A2 antibody, ASA-B0172,IHC(P)
Mouse Lung Tissue
Mouse Lung Tissue
Anti- ATP2A2 antibody, ASA-B0172,IHC(P)
Rat Lung Tissue
Rat Lung Tissue