More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| ataxin 3 | Polyclonal | IgG | Rabbit | Human, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Ataxin 3 (226-254aa EEDLQRALALSRQEIDMEDEEADLRRAIQ), different from the related mouse and rat sequences by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ATXN3 |
Protein | Ataxin-3 |
Uniprot ID | P54252 |
Function | Deubiquitinating enzyme involved in protein homeostasis maintenance, transcription, cytoskeleton regulation, myogenesis and degradation of misfolded chaperone substrates. Binds long polyubiquitin chains and trims them, while it has weak or no activity against chains of 4 or less ubiquitins. Involved in degradation of misfolded chaperone substrates via its interaction with STUB1/CHIP: recruited to monoubiquitinated STUB1/CHIP, and restricts the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension. In response to misfolded substrate ubiquitination, mediates deubiquitination of monoubiquitinated STUB1/CHIP. Interacts with key regulators of transcription and represses transcription: acts as a histone- binding protein that regulates transcription. |
Tissue Specificity | Ubiquitous. |
Sub-cellular localization | Nucleus matrix. |
Sequence Similarities | Contains 1 Josephin domain. |
Aliases | AT3 antibody|Ataxin 3 antibody|Ataxin-3 antibody|ATX3 antibody|ATX3_HUMAN antibody|ATXN3 antibody|EC 3.4.22. antibody|JOS antibody|Josephin antibody| Machado Joseph disease (spinocerebellar ataxia 3, olivopontocerebellar ataxia 3, autosomal dominant, ataxin 3) antibody|Machado Joseph disease antibody| Machado Joseph disease protein 1 antibody|Machado-Joseph disease protein 1 antibody|Machado-Joseph disease protein 1 homolog antibody|MJD antibody| MJD gene antibody|MJD1 antibody|Olivopontocerebellar ataxia 3 antibody| OTTHUMP00000221583 antibody|OTTHUMP00000221585 antibody| OTTHUMP00000221586 antibody| OTTHUMP00000221587 antibody| OTTHUMP00000231995 antibody|OTTHUMP00000231997 antibody|Rsca3 antibody|SCA3 antibody|SCA3 gene antibody|Spinocerebellar ataxia type 3 protein antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Ataxin 3 antibody, ASA-B0162, Western blotting
All lanes: Anti Ataxin 3 (ASA-B0162) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted band size: 42KD
Observed band size: 42KD
All lanes: Anti Ataxin 3 (ASA-B0162) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted band size: 42KD
Observed band size: 42KD
Anti- Ataxin 3 antibody, ASA-B0162, IHC(P)
Human Lung Cancer Tissue
Human Lung Cancer Tissue