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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| ataxin 1 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Ataxin 1 (778-808aa ESRKLEKSEDEPPLTLPKPSLIPQEVKICIE), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ATXN1 |
Protein | Ataxin-1 |
Uniprot ID | P54253 |
Function | Chromatin-binding factor that repress Notch signaling in the absence of Notch intracellular domain by acting as a CBF1 corepressor. Binds to the HEY promoter and might assist, along with NCOR2, RBPJ-mediated repression. Binds RNA in vitro. May be involved in RNA metabolism. |
Tissue Specificity | Widely expressed throughout the body. |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | Belongs to the ATXN1 family. |
Aliases | 6Ataxin-1 antibody|ATX1 antibody|ATX1_HUMAN antibody|Atxn1 antibody| D6S504E antibody| OTTHUMP00000016065 antibody|SCA1 antibody| Spinocerebellar ataxia type 1 protein antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Ataxin 1 antibody, ASA-B0160, Western blotting
All lanes: Anti Ataxin 1 (ASA-B0160) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Lane 3: PANC Whole Cell Lysate at 40ug
Predicted band size: 87KD
Observed band size: 87KD
All lanes: Anti Ataxin 1 (ASA-B0160) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Lane 3: PANC Whole Cell Lysate at 40ug
Predicted band size: 87KD
Observed band size: 87KD
Anti- Ataxin 1 antibody, ASA-B0160, IHC(P)
Human Lung Cancer Tissue
Human Lung Cancer Tissue