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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| aryl hydrocarbon receptor | Polyclonal | IgG | Rabbit | Mouse | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of mouse Aryl hydrocarbon Receptor(497-511aa EAALKHEQIGHAQDV). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | AHR |
Protein | Aryl hydrocarbon receptor |
Uniprot ID | P30561 |
Function | Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues. Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1. Inhibits PER1 by repressing the CLOCK-ARNTL/BMAL1 heterodimer mediated transcriptional activation of PER1. |
Tissue Specificity | Expressed in all tissues tested including brain, heart, kidney, liver, lung, muscle, ovary, skin, spleen and thymus. |
Sub-cellular localization | Cytoplasm. Nucleus. Note: Initially cytoplasmic; upon binding with ligand and interaction with a HSP90, it translocates to the nucleus. |
Sequence Similarities | Contains 1 bHLH (basic helix-loop-helix) domain. |
Aliases | Ah receptor antibody|AHR antibody|AHR_HUMAN antibody|Aromatic hydrocarbon receptor antibody|Aryl hydrocarbon receptor antibody|Aryl hydrocarbon receptor precursor antibody|bHLHe76 antibody|Class E basic helix loop helix protein 76 antibody|Class E basic helix-loop-helix protein 76 antibody|HGNC:348 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Aryl hydrocarbon Receptor antibody, ASA-B0156, Western blotting
Lane 1: Mouse Brain Tissue Lysate
Lane 2: Mouse Heart Tissue Lysate
Lane 3: Mouse Liver Tissue Lysate
Lane 1: Mouse Brain Tissue Lysate
Lane 2: Mouse Heart Tissue Lysate
Lane 3: Mouse Liver Tissue Lysate
Anti-Aryl hydrocarbon Receptor antibody, ASA-B0156, IHC(P)
Mouse Cardiac Muscle Tissue
Mouse Cardiac Muscle Tissue
Anti-Aryl hydrocarbon Receptor antibody, ASA-B0156, IHC(P)
Mouse Brain Tissue
Mouse Brain Tissue