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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cytochrome P450, family 19, subfamily A, polypeptide 1 | Polyclonal | IgG | Rabbit | Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of rat Aromatase(455-476aa LKRFHVKTLQKRCIENMPKNND). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CYP19A1 |
Protein | Cytochrome P450 19A1 |
Uniprot ID | P22443 |
Function | Catalyzes the formation of aromatic C18 estrogens from C19 androgens. |
Tissue Specificity | |
Sub-cellular localization | Membrane; Peripheral membrane protein. |
Sequence Similarities | |
Aliases | ARO 1 antibody|ARO antibody|ARO1 antibody|Aromatase antibody|CP19A_HUMAN antibody|CPV antibody|CPV1 antibody|CYAR antibody|CYP19 antibody|CYP19A1 antibody|CYPXIX antibody|Cytochrome P-450AROM antibody|Cytochrome P450 19A1 antibody|Cytochrome P450 family 19 subfamily A polypeptide 1 antibody|Cytochrome P450, family 19, subfamily A, polypeptide 1 antibody|Cytochrome P450, subfamily XIX(aromatization of androgens) antibody|Estrogen synthase antibody|Estrogen synthetase antibody|Flavoprotein linked monooxygenase antibody|MGC104309 antibody|Microsomal monooxygenase antibody|OTTHUMP00000162543 antibody|OTTHUMP00000198350 antibody|P 450AROM antibody|P450AROM antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Aromatase antibody, ASA-B0153, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Thymus Tissue Lysate
Lane 3: Rat Kidney Tissue Lysate
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Thymus Tissue Lysate
Lane 3: Rat Kidney Tissue Lysate
Anti-Aromatase antibody, ASA-B0153, IHC(P)
Rat Brain Tissue
Rat Brain Tissue