More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| arginase, type II | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Arg2 (67-85aa DFGDLSFTPVPKDDLYNNL), different from the related mouse sequence by three amino acids and from the related rat sequence by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ARG2 |
Protein | Arginase-2, mitochondrial |
Uniprot ID | P78540 |
Function | May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to NO synthase. Since NO synthase is found in the penile corpus cavernosum smooth muscle, the clitoral corpus cavernosum and the vagina, arginase II plays a role in both male and female sexual arousal. It is therefore a potential target for the treatment of male and female sexual arousal disorders. |
Tissue Specificity | Expressed most strongly in kidney and prostate, much less strongly in the brain, skeletal muscle, placenta, lung, mammary gland, macrophage, uterus, testis and gut, but apparently not in the liver, heart and pancreas. |
Sub-cellular localization | Mitochondrion. |
Sequence Similarities | Belongs to the arginase family. |
Aliases | ARG2 antibody|ARGI2_HUMAN antibody|Arginase II mitochondrial antibody|Arginase type II antibody|Arginase-2 antibody|arginase-2, mitochondrial antibody|Kidney arginase antibody|Kidney type arginase antibody|Kidney-type arginase antibody|L arginine amidinohydrolase antibody|L arginine ureahydrolase antibody|mitochondrial antibody|Non hepatic arginase antibody|Non-hepatic arginase antibody|Nonhepatic arginase antibody|Type II arginase antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Arg2 antibody, ASA-B0150, Western blotting
Lane 1: Rat Lung Tissue Lysate
Lane 2: Rat Testis Tissue Lysate
Lane 3: A549 Cell Lysate
Lane 4:293T Cell Lysate
Lane 1: Rat Lung Tissue Lysate
Lane 2: Rat Testis Tissue Lysate
Lane 3: A549 Cell Lysate
Lane 4:293T Cell Lysate
Anti-Arg2 antibody, ASA-B0150, IHC(P)
Human Mammary Cancer Tissue
Human Mammary Cancer Tissue