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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| aquaporin 4 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 4 (295-323aa KPGVVHVIDVDRGEEKKGKDQSGEVLSSV), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | AQP4 |
Protein | Aquaporin-4 |
Uniprot ID | P55087 |
Function | Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system. |
Tissue Specificity | Brain - muscle >> heart, kidney, lung, and trachea. |
Sub-cellular localization | Membrane; Multi-pass membrane protein. |
Sequence Similarities | Belongs to the MIP/aquaporin (TC 1.A.8) family. |
Aliases | AQP 4 antibody|AQP-4 antibody|AQP4 antibody|AQP4_HUMAN antibody| Aquaporin type 4 antibody| Aquaporin-4 antibody|Aquaporin4 antibody|HMIWC 2 antibody|HMIWC2 antibody| Mercurial insensitive water channel antibody| Mercurial-insensitive water channel antibody|MGC22454 antibody|MIWC antibody| WCH 4 antibody|WCH4 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Mouse, Rat Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Aquaporin 4 antibody, ASA-B0140, Western blotting
All lanes: Anti Aquaporin 4 (ASA-B0140) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Predicted band size: 35KD
Observed band size: 45KD
All lanes: Anti Aquaporin 4 (ASA-B0140) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Predicted band size: 35KD
Observed band size: 45KD
Anti- Aquaporin 4 antibody, ASA-B0140, IHC(P)
Mouse Brain Tissue
Mouse Brain Tissue
Anti- Aquaporin 4 antibody, ASA-B0140, IHC(P)
Rat Brain Tissue
Rat Brain Tissue