More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| aquaporin 3(Gill blood group) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 3(278-292aa EENVKLAHVKHKEQI), different from the related rat and mouse sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | AQP3 |
Protein | Aquaporin-3 |
Uniprot ID | Q92482 |
Function | Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism (By similarity). |
Tissue Specificity | Widely expressed in epithelial cells of kidney (collecting ducts) and airways, in keratinocytes, immature dendritic cells and erythrocytes. Isoform 2 is not detectable in erythrocytes at the protein level. |
Sub-cellular localization | Basolateral cell membrane; Multi-pass membrane protein. Note: In collecting ducts of kidney. |
Sequence Similarities | Belongs to the MIP/aquaporin (TC 1.A.8) family. |
Aliases | AQP 3 antibody|AQP-3 antibody|Aqp3 antibody|AQP3_HUMAN antibody|Aquaglyceroporin-3 antibody|Aquaporin 3(GIL blood group) antibody|Aquaporin 3(Gill blood group) antibody|Aquaporin-3 antibody|Aquaporin3 antibody|GIL antibody|Gill blood group antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Aquaporin 3 antibody, ASA-B0137, Western blotting
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Rat Lung Tissue Lysate
Lane 3: Mouse Kidney Tissue Lysate
Lane 4: M453 Cell Lysate
Lane 5: MMC Cell Lysate
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Rat Lung Tissue Lysate
Lane 3: Mouse Kidney Tissue Lysate
Lane 4: M453 Cell Lysate
Lane 5: MMC Cell Lysate
Anti-Aquaporin 3 antibody, ASA-B0137, IHC(P)
Rat Kidney Tissue
Rat Kidney Tissue
Anti-Aquaporin 3 antibody, ASA-B0137, ICC
HELA Cells
HELA Cells