More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
| A synthetic peptide corresponding to a sequence at the N-terminus of human APPL(138-158aa NDHDAAINRYSRLSKKRENDK), identical to the related mouse and rat sequences. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | APPL1 |
Protein | DCC-interacting protein 13-alpha(Dip13-alpha) |
Uniprot ID | Q9UKG1 |
Function | Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction. |
Tissue Specificity | High levels in heart, ovary, pancreas and skeletal muscle. |
Sub-cellular localization | Early endosome membrane; Peripheral membrane protein. Nucleus. Note: Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF. |
Sequence Similarities | Contains 1 PH domain. |
Aliases | Adapter protein containing PH domain antibody|Adaptor Protein Containing pH Domain antibody|Adaptor protein containing pH domain PTB domain and leucine zipper motif 1 antibody|Adaptor protein containing pH domain PTB domain and leucine zipper motif antibody|AKT2 INTERACTOR antibody|APPL 1 antibody|APPL1 antibody|DCC interacting protein 13 alpha antibody|DCC-interacting protein 13-alpha antibody|DIP 13 alpha antibody|DIP13 alpha antibody|Dip13-alpha antibody|DIP13alpha antibody|DP13A_HUMAN antibody|KIAA1428 antibody|PTB domain and leucine zipper motif 1 antibody|PTB Domain and Leucine Zipper Motif antibody|Signaling adaptor protein DIP13alpha antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-APPL antibody, ASA-B0129, Western blotting
HELA Cell Lysate
