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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| clusterin | Polyclonal | IgG | Rabbit | Human | ELISA, IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E. coli-derived human Apolipoprotein J recombinant protein (Position: S228-E449). Human Apolipoprotein J shares 76.1 % and 75.2 % amino acid (aa) sequence identity with mouse and rat Apolipoprotein J, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CLU |
Protein | Clusterin |
Uniprot ID | P10909 |
Function | Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress- induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. |
Tissue Specificity | Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung. |
Sub-cellular localization | Isoform 1: Secreted. Note: Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. |
Sequence Similarities | Belongs to the clusterin family. |
Aliases | 40 antibody|AAG 4 antibody|AAG4 antibody|Aging associated protein 4 antibody|Aging-associated gene 4 protein antibody|AI893575 antibody| APO J antibody|Apo-J antibody|APOJ antibody|ApoJalpha antibody|ApoJbeta antibody|Apolipoprotein J antibody|ApolipoproteinJ antibody|CLI antibody|CLU antibody|CLU1 antibody|CLU2 antibody|CLUS_HUMAN antibody|Clusterin alpha chain antibody|clusterin antibody| Clusterin beta chain antibody|Clusterin precursor antibody|Complement associated protein SP 40 40 antibody|Complement associated protein SP 40 antibody|Complement associated protein SP40 antibody|Complement cytolysis inhibitor a chain antibody|Complement cytolysis inhibitor antibody|Complement cytolysis inhibitor b chain antibody|Complement lysis inhibitor antibody|Complement-associated protein SP-40 antibody| D14Ucla3 antibody|Dimeric acid glycoprotein antibody|Glycoprotein 80 antibody|Glycoprotein III antibody|GP80 antibody|Ku70-binding protein 1 antibody|KUB 1 antibody|KUB1 antibody|MGC24903 antibody|NA1/NA2 antibody|RATTRPM2B antibody|SGP 2 antibody|SGP2 antibody|SP 40 antibody|SP40 antibody|Sufated glycoprotein 2 antibody|Sugp-2 antibody|Sulfated glycoprotein 2 antibody|Testosterone repressed prostate message 2 antibody|Testosterone-repressed prostate message 2 antibody|TRPM 2 antibody|TRPM-2 antibody|TRPM2 antibody|TRPM2B antibody|Trpmb antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| ELISA | 0.1-0.5μg/ml | Human | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Apolipoprotein J antibody, ASA-B0124, Western blot
All lanes: Anti Apolipoprotein J (ASA-B0124) at 0.5ug/ml
Lane 1: SKOV Whole Cell Lysate at 40ug
Lane 2: U87 Whole Cell Lysate at 40ug
Lane 3: PANC Whole Cell Lysate at 40ug
Predicted band size: 52KD
Observed band size: 82KD
Lane 1: SKOV Whole Cell Lysate at 40ug
Lane 2: U87 Whole Cell Lysate at 40ug
Lane 3: PANC Whole Cell Lysate at 40ug
Predicted band size: 52KD
Observed band size: 82KD
Anti- Apolipoprotein J antibody, ASA-B0124, IHC(P)
Human Testis Tissue
Human Testis Tissue