More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| apolipoprotein E | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Apolipoprotein E recombinant protein (Position: K19-H317). Human Apolipoprotein E shares 73% and 72% amino acid (aa) sequence identity with mouse and rat Apolipoprotein E, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | APOE |
Protein | Apolipoprotein E |
Uniprot ID | P02649 |
Function | Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. |
Tissue Specificity | Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL). APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, adrenal, ovary, kidney and muscle. |
Sub-cellular localization | Secreted. |
Sequence Similarities | Belongs to the apolipoprotein A1/A4/E family. |
Aliases | AD2 antibody|Alzheimer disease 2 antibody|Apo E antibody|Apo-E antibody|ApoE antibody|APOE_HUMAN antibody|APOEA antibody|Apolipoprotein E antibody|Apolipoprotein E3 antibody|ApolipoproteinE antibody|Apoprotein antibody|LDLCQ5 antibody|LPG antibody|MGC1571 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Apolipoprotein E antibody, ASA-B0122, Western blotting
Anti Apolipoprotein E (ASA-B0122) at 0.5ug/ml
Human Placenta Tissue Lysate at 50ug
Predicted band size: 36KD
Observed band size: 36KD
Anti Apolipoprotein E (ASA-B0122) at 0.5ug/ml
Human Placenta Tissue Lysate at 50ug
Predicted band size: 36KD
Observed band size: 36KD
Anti- Apolipoprotein E antibody, ASA-B0122, IHC(P)
Human Placenta Tissue
Human Placenta Tissue